The application of the CRISPR-Cas9 system for mammalian genome editing was reported first in 2013, by Zhang and separately by George Church at Harvard university.
But now, in a landmark study by George Church and his team at the Wyss Institute for Biologically Inspired Engineering at Harvard university and Harvard Medical school, the gene editing system known as CRISPRAS9 has been used to genetically engineer pig DNA
Church is the Robert Winthrop Professor of Genetics at Harvard Medical school and a Wyss core faculty member.
The advance, reported by Church and his team, including the study lead author, Luhan Yang, a research fellow at HMS and the Wyss, was published in the October 11 issue of Science.
or PERVSROUGHT more than a billion dollarsworth of pharmaceutical industry investments in developing xenotransplant methods to a standstill by the early 2000s, said Church.
Church and his team have inactivated all 62 repetitive genes containing a PERV in pig DNA,
and is currently collaborating with Church on further genetic modifications of his pigs. f Church and his team are able to produce pigs from genetically engineered embryos lacking PERVS by the use of CRISPR-Cas9,
Then scientists placed the cells into culture conditions containing certain cytokines and soon saw that their efforts were continued successful cells to divide as stem cells instead of undergoing differentiation into B-cells.
In a culture without these cytokines the cells differentiated into various immune cells. Team wanted to test
The carbonic anhydrase would be immobilized with solvent inside a reactor vessel that serves as a large purification column.
he CPM+LPCS that we have derived from human ipscs can be transferred from one medium to another for continuous culture
and smuggle cancer signals their neighbors New research in The FASEB Journal suggests that an in vitro co-culture system robustly quantifies the transfer of fluorescent proteins between cells
Harvard university scientist George Church and his former student, Farren Isaacs, of Yale, held a press conference to announce a breakthrough of their own.
Isaacs and Church described how, in separate experiments, they created what they called a enomically reordered organism, or GRO.
Church and Isaacs recently announced they had formed a Boston-based company, enevolv, to supply such GROS for use by industry to,
Church declared that e are aiming at modifying plant and animal cells, and maybe plants and animals. potential objection to the technology is already apparent:
Church likewise argues that his GROS are safe because they depend upon unnatural amino acids that are supplied only in the lab. Withhold these and the bacteria die.
Both Romesberg and Church reported a tiny fraction of the bacteria managed to slip the genetic handcuffs via mutation.
The article also touches upon the misogyny that possibly exists within the modern dating culture.
"but rather in the midst of reviving the dating culture by building something that is changing the world"and leading to"meaningful relationships."
"While reading the recent Vanity fair article about today's dating culture, we were saddened to see that the article didn't touch upon the positive experiences that the majority of our users encounter daily,
Turkey's journey towards a new startup culture While far from the levels of Greece, Spain,
because the app will red-flag the issue, the doctor can intervene, and the doctor and patient can work on solutions together.
In fact, Carme Ruscalleda, the Catalan chef behind the three Michelin starred restaurant Sant Pau, used it to make an edible model of one of the stained-glass windows of Santa maria del Mar, a gothic church in Barcelona."
"We have to establish a culture of food machine usage. Cooks are keen to do demonstrations
or not the new finding spells trouble for modern civilization it likely will help researchers understand how
The Capflank-method is suited even for heavily fragmented ancient DNA extracted from animal bones from museum collections.
Greenwood's colleagues successfully applied Capflank to samples from koalas kept in museums. Capflank is at its most efficient though with fresh DNA.
and practice cultures across our health system increases the generalizability of our findings to other health care settings.
The researchers studied the squeezed B cells in culture and found that they could expand antigen-specific T cells at least as well as existing methods using antibody-coated beads.
On either face of a silicon wafer, they etch dense arrays of tiny rectangular columns tens of micrometers across
The task force turned to crowdsourcing to develop a design for the memorial to the passengers and crew of Flight 93.
richest companies abandoned this idea as a red flag. Not Feedly, Digg, AOL, Newsblur, and others,
where the length of the mercury column in a capillary is controlled by the thermal expansion of the mercury in a reservoir connected to the capillary.
Tuning the voltage allowed them to control the length of the metal column in the capillary.
but let them experience the hacker culture, and collaborate with really different thinkers of this world,
The Sony hack has displayed a lot about the failing of our modern, wired corporate culture. Like just how casual internal company HR employees treat email communication, for example.
but also raise red flags, it makes more sense than ever for tech startups to adopt a policy of radical openness:
A company that starts out with a closed paradigm and later chooses to open up will have a tough time rerouting company culture
and director David Fincher and actress Robin Wright both brought home a statue for their work.
it also planting the flag for an entire ecosystem of startup companies engaged in overturning the ways in
and contributes weekly to art & culture magazine Beautiful/Decay. With online sales up 17%and physical retail down 11%,it no wonder retailers large and small are increasingly living online.
and a robust arts culture and one day we hope to be able to bring their work to our global audience of over 57 million members.
More importantly, deep fragmentation between language and cultures meant humans from different backgrounds were unable to effectively communicate with each other.
Wide adoption of a set of unicode ideograms helped people of different cultures to communicate with one another
Nevertheless with a population of 144 million people and a strong tech culture the country represents a lot of emerging market opportunity for Apple (and others.
which you apply to your temple and neck. When you open Thync app, it quickly connects to the module
Instead, it uses tiny pulses of electricity to stimulate the skin at your temple, which then activates the instinctual fight-or-flight response in your brain to indirectly affect emotional response.
and I really wanted a simple way to send donations to our local church, which typically collected cash
Our culture is trained to think about a person with an unusual body or mind as weak Herr says.
a Dutch social psychologist who developed ways to measure a set of cultural dimensions for comparing cultures.
It shows that differences in culture affect the dynamics of social spreading processes in a measurable way.
whose cultures differ in how they value individualism versus collectivism, say Lang and co. What more,
scientists from Harvard university, led by George Church, say theye created big advances to the method which people need to start worrying about.
In museums it could pull up Wikipedia articles when focused on a piece of art (Amazon says that it will add image-recognition for artwork to Firefly later in the year).
As a practical matter, it will also offer museum restoration artists and others a way to put faces to long-dead victims
. or museums, there is no other way to do itou aren going to ship a 2, 000 year old Egyptian mummy in the mail,
That fountain of variability could be key to finding ways to push our fermenting friends to more efficiently create biofuels and other chemicals f
Thanks to its privacy laws and discreet culture, the country is emerging as a hub for advanced security technology.
The findings were announced by Tata Memorial Centre's Dr Anil D'Cruz at an ongoing meeting of American Society of Clinical Oncology at Chicago on May 31.
The findings were announced by Tata Memorial Centre's Dr Anil D'Cruz at an ongoing meeting of American Society of Clinical Oncology at Chicago on May 31.
Users stick the device, called Thync, onto their front temple. A second connected pad goes in a spot farther back on the head,
The group of international researchers include engineers from the Visual Computing Research center, Tel-aviv University, the Memorial University of Newfoundland, the University of Konstaz and Shandong University.
they can actually 3d print life-sized human statues. Envisioning a wide variety of applications, the Israeli developers have emphasized just its potential by 3d printing an entire car body in a single piece.
and almost immediately identifying dangerous bacteria at the bedside much faster than the days it normally takes to grow or"culture"the microbes in the laboratory from a patient's blood,
"We were able to demonstrate that we could make this scaffold and culture cells within it,
if it weren for the unique culture at UC San diego that fosters entrepreneurship. hat one of the reasons
which is a one-molecule-thick bag capable of holding liquid within it during exposure to the ultra high vacuum of the electron microscope column.
the IMS researchers have integrated an individual readout chain on the chip for each pixel column.
In each pixel column, the three colors red, green and blue are recorded at the same time and over the entire pixel area.
#Scott & White Memorial Implants Miniaturized, Wireless Monitoring Sensor to Help Manage Heart failure Scott & White Memorial is one of six hospitals in Texas
and the first hospital in the Baylor Scott & White Health system to offer the device.
Director for Advanced Heart failure at Scott & White Memorial. his device will give us the ability to anticipate problems with our patients before they occur,
cardiologist at Scott & White Memorial. e think that we can provide significantly improved quality of life by partnering with the patient in acting preventatively as opposed to responding
In between each layer are vertical submicroscopic columns, which connect the points at which the wires crisscross.
Each of these columns contains: a"memory cell, "which can store a single bit of data.
"Could eventually replace animal models Modeling early heart development is difficult to achieve in a petri dish and tissue culture plates,
and neural progenitors. he idea is coated culture vessels with the polymer are arranged into arrays or factories each capable of supporting the production of billions of human pluripotent stem cells for applications in regenerative medicine and transplants.
and neural progenitors. he idea is coated culture vessels with the polymer are arranged into arrays or factories each capable of supporting the production of billions of human pluripotent stem cells for applications in regenerative medicine and transplants.
that we can culture the entire construct over prolonged periods of time, and that we can repopulate the vascular system and musculature.
At the same time, populations of muscle and vascular cells were being grown in culture. The research team then cultured the forelimb matrix in a bioreactor, within
After five days in culture, electrical stimulation was applied to the potential limb graft to further promote muscle formation, and after two weeks
and small genomes, may be the reason they can be grown in lab culture: they are stripped-down life forms with the barebones requirement of genes,
because some organisms detected widely in the environment fail to grow in culture like other bacteria.
though only 29 have representatives that will grow in culture. The new discovery allowed the team not only to define about a third of all bacterial phyla but, thanks to the nearly complete genomes
#New Single-use Harvesting Technology for High Cell Density Cultures Sartorius Stedim Biotech (SSB), a leading international supplier for the biopharmaceutical industry, has introduced Sartoclear Dynamics,
a clarification system featuring new single-use technology for harvesting mammalian cell cultures with high cell densities.
DE can be mixed directly into the cell culture fluid. This porous filter aid prevents blockage of the filters.
The ready-to-use clarification system enables harvesting of high cell density cultures up to 2, 000 liters 0
Could eventually replace animal models Modeling early heart development is difficult to achieve in a petri dish and tissue culture plates,
but right to where the fruit column started to bear, because theye heavy. Many of the papaya fields are on leasehold land in or near Kapoho,
or by column heading, but you can't do a lot of deep searching. And usually you could only access the database from a Windows computer running an outdated version of Internet explorer,
Sending push notifications from a museum about an app that provides more information about exhibits
living in culture, these cells are not suitable for hair transplants, since they lost their ability to induce follicle formation.
seeded into a 3d scaffold and nourished with a culture gel full of nutrients to encourage growth.
Now experts and volunteers are recreating history to digitally rebuild lost artefacts using photos taken in museums
Phd students Chance Coughenour and Matthew Vincent, from the Initial Training Network for Digital Cultural heritage (ITN-DCH) came up with the idea of reconstructing objects digitally from crowd sourced images days after ISIS
militants reportedly smashed artworks in the Mosul Museum in Iraq. They use photogrammetry a technique that uses software to transform 2d photos into 3d images as well as photos from the public, the BBC reported.
which once stood in the museum, was clearly once popular with visitors, as the volunteers had 16 images to work from. he more photographs you have,
In 2003, the National Museum of Iraq was looted during the invasion, and widespread looting of sites such as the Palace of Ashurnasipal II at Nimrud occurred,
Palaeontologists found the rare soft tissue on eight dinosaur fossils that have spent more than a century in storage at the Natural history Museum in London.
but just 29 have species that will grow in laboratory cultures. The team has named now 20 the new phyla they identified after award-winning microbiologists
'Modeling early heart development is difficult to achieve in a petri dish and tissue culture plates,
George Church, a genetics professor at Harvard Medical school oversaw one of the studies that was published Wednesday in the journal Nature,
s AI going to destroy civilization as we know it? It not just people on the street, Christian says. t also true for a lot of the people at the very places developing these technologies. hristian cites the example of Stuart Russell, a professor of computer science at the University of California, Berkeley,
The first breach has been linked to earlier thefts of personal data from millions of records at Anthem Inc, the second largest U s. health insurer,
and collaborative in ways that rivaled---if not beat--the work cultures of other great companies
a massive talent pool to explore for great culture and skill fit, says Jake Goldman,
5 Essential Keys to Leading a Remote Workforcetraditional companies foster company culture mainly through in-person activities.
and develop remote company cultures. Vadun, of Fireenginered, says the company has virtual book and walking clubs.
Origin Eight, a web and application development company with 25 remote workers, takes time to build culture.
And it takes leadership to further the culture, says CEO and founder Seth Viebrock. In-person meetings are still valuable,
says Emily Tetto, director of talent and culture. Scrapinghub, a data and information extraction company with 107 remote workers, focuses on motivation over productivity.
Company culture is vital to hiring and retaining workers. Switching management techniques to focus less on face time and more on goals and results would increase productivity at every company.
000 residents in Fort Collins, Colorado, and neighboring communities who rely on the Cache la Poudre River watershed as their water source.
The neighboring forests surrounding Fort Collins catch and filter the water. Thus, if the forests are harmed,
This year on World Environment Day, Anheuser-busch employees in Fort Collins, Los angeles and across the United states will honor these water journeys
or sculpt a clay statue using both hands. HP and Zspace are positioning the display primarily for science
According to a report in the News Scientist, Modern face-recognition algorithms are so good theye already found their way into social networks, shops and even churches.
#Health insurer Anthem says database of customer employee info hacked Anthem, the second-largest health insurance company in America, said late Wednesday that a database containing personal information of approximately 80 million of its customers
though Anthem said it was likely that"tens of millions"of records were stolen. Anthem also said the breach exposed names, addresses, birthdates, Social security numbers,
email addresses and employment details--including income. However the insurer did say that credit card information was compromised not
Anthem said it had detected the breach itself and would notify affected customers via letter and e-mail.
Matt Cullina, CEO of identity protection specialist IDT911, told Foxnews. com that the Anthem hack highlights the importance of extensive cyber security planning. ith high legal costs and the risk
Real termites In Africa, millions of the tiny insects work together to build very large mounds of soil for their underground nests, facing enormous challenges over the year or so of construction.
and Obama, promoted"vulgar culture, "committed fraud by pretending to be Communist party officials or agitated for separatist causes.
It can also be broken down individually, by row and by column. orpheesis another prototype, which uses flexible mobile devices made with Lycra or alloy displays.
and colleague, Woodring E. Wright, M d.,Ph d.,professor of cell biology and internal medicine, found that 6-thio-2'-deoxyguanosine could stop the growth of cancer cells in culture and decrease the growth of tumors in mice.
and membership and professional societies. takeholders in clinical trials should foster a culture in which data sharing is expected the norm,
#Pall Acquires Innovative Acoustic Wave Separation Technology Pall obtained an exclusive license for acoustic wave separation (AWS), a disruptive technology from Flodesign Sonics for cell culture clarification for both
The technology will complement Pall's STAXTM depth filtration products to enable continuous clarification of cell culture, enabling integration of the bioreactor with downstream processing
When a cell culture passes through the flow channel, the cells are trapped at the nodes of the acoustic waves
"The AWS products will eliminate the reliance on centrifugation for cell culture clarification, and will provide a continuous feed stream for direct integration with the Biosmb platform.
Pall plans to introduce a single-use product line using AWS technology for the clarification of cell culture at both bench scale
"It works in a wide range of cancers both in culture and in human tumors developing in animal models,"explained Dr. Burris."
#Biopharma Demand Is Driving the Cell Culture Market The production of biologic therapies such as vaccines, blood factors,
and monoclonal antibodies is based on cell culturend the products that facilitate cell culture. According to Kalorama Information, these products,
The demand for cell culture and related products is not just rising, but accelerating, a phenomenon that is reflected in the timing of biopharmaceutical product launches:
The market for cell cultures is expected to grow rapidly alongside biopharmaceuticals and with significant evolution in culture technology.
Bacterial culture is the workhorse of the biotechnology industry. Animal cells are much more fragile than yeast and bacterial cells.
Animal cell culture is complicated more; thus, it is more expensive than traditional fermentation. The most widely used animal cells are Chinese hamster ovary (CHO) cells.
and thus are easier to grow in culture. CHO cells are epithelial cells that were introduced to science in the 1950s.
and grow well in culture. Cell Culture Media Trends The increasing use of mammalian cell lines,
including versatile and uniquely capable stem cells, in upstream bioprocessing has imposed specific demands on cell cultures:
namely, better-defined media. The rising stakes and production levels of companies active in biopharmaceuticals have dictated improved reproducibility or consistency in product;
and the use of culture product more amenable to downstream processing or purification. Media can be organized into three broad types:
The biopharmaceutical industry shift away from animal-derived culture products is expected to continue, particularly given the ascension of mammalian cell lines in biopharmaceutical production.
Working and Master Cell Banks Once a biopharmaceutical company obtains a beginning cell bank from a cell culture collection,
Shifts in Productive Capacity Cell culture production is growing faster in the Asia Pacific region than any place else in the world.
New technologies used in cell culture production, in particular single-use disposable production systems, are dramatically lowering building costs of biomanufacturing plants.
Market Drivers An important driver of the cell culture market is the production of seasonal influenza vaccines,
However, this labor-intensive approach to vaccine development is currently being replaced by cell-culture systems.
Prefluce, the first cell culture-based vaccine, received European approval in March 2011 and was available for the 2011012 influenza season in the 13 participating European union countries.
which is the first U s.-licensed (trivalent inactivated) influenza vaccine manufactured using cell culture technology.
Stem cell research will also add to the robust growth of the cell culture market. The growing use
and keeps blast-damaged walls from turning into a hail of flying debris. Boone says that the wallpaper has undergone already blast testing at Fort Polk, Louisiana, and Eglin Air force base,
the word changed from blue to red. a plastic dish imprinted with BMP-2 proteins that stimulate bone growth to control the direction of tissue growth. sodium ampicillin printed on a bacterial culture printed to test the effectiveness of a topographical distribution of the antibiotic
Just last month at the International Society for Stem Cell Research conference in Stockholm, scientists reported updated methods to more reproducibly culture organoids with better architecture.
Rather, the clock is a visual metaphor to warn the public about how close the world is to a potentially civilization-ending catastrophe.
and smooth muscles cells and having the cells culture for a period of up to two weeks in a static bioreactor.
which was named recently esign of the Year"by London Design Museum, is called organs-on-chips
On either face of a silicon wafer, they etch dense arrays of tiny rectangular columns tens of micrometers across
A scanning electron micrograph of the new microfiber emitters, showing the arrays of rectangular columns etched into their sides.
On either face of a silicon wafer, they etch dense arrays of tiny rectangular columns tens of micrometers across
A scanning electron micrograph of the new microfiber emitters, showing the arrays of rectangular columns etched into their sides.
"In the past, people have used impedance spectroscopy for cell cultures or relatively simple measurements in tissue. What makes this unique is extending that to detect
and destroying norovirus Hansman's research team recently discovered that a"nanobody"called Nano-85 was able to bind to intact norovirus-like particles (VLPS) in culture.
May 19th, 2015nnco and Museum of Science fiction to Collaborate on Nanotechnology and 3d printing Panels at Awesome Con May 19th, 201 0
Uncovering the real history of art using a graphene scanner Museum curators, art restorers, archaeologists and the broader public will soon be able to learn much more about paintings and other historic objects,
and tablet app to be exploited by local and regional museums. The app is currently being trialled at one of the partners
the Asturias Fine art Museum in Oviedo. It shows the different layers of the painting the visitor is looking at
Marta Flórez, of the Asturias Fine art Museum, explained: sing the prototype, we have been able to distinguish clearly between different pigments,
The prototype is also being validated with some recently unearthed 3rd century pottery from the Stara Zagora regional history museum in Bulgaria.
When the project ends in December 2015, one of the options the consortium is assessing is putting this cost-effective solution at the service of smaller local and regional museums without art restoration departments so that they too,
like the bigger museums, can make important discoveries about their collections c
#Researchers develop new way to manufacture nanofibers Abstract: Researchers at the University of Georgia have developed an inexpensive way to manufacture extraordinarily thin polymer strings commonly known as nanofibers.
Culture, Sports, Science and Technology (MEXT) E
#Leti Demos New Process to Fabricate High-brightness Micro-LED Arrays for Next-gen Head-mounted and Head up displays:
"We estimate yields on the order of grams per liter from batch cultures under optimized conditions,
Now, results of a study led by Johns Hopkins investigators suggests that a device composed of a magnetic column paired with custom-made magnetic nanoparticles may hold a key to bringing immunotherapy into widespread and successful clinical use.
They then ran the plasma through a magnetic column. The tumor-fighting T cells bound to aapcs and stuck to the sides of the column,
while other cells washed straight through and were discarded. The magnetic field of the column activated the T cells,
which were washed then off into a nourishing broth, or culture, to grow and divide. After one week, their numbers had expanded by an estimated 5, 000 to 10,000 times.
Because numbers of these cells could be expanded quickly enough to be therapeutically useful the approach could open the door to individualized immunotherapy treatments that rely on a patient's own cells,
"The aapcs and magnetic column together provide the foundation for simplifying and streamlining the process of generating tumor-specific T cells for use in immunotherapy,
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