| Cryo electron microscopy (3) |  |
| Structural biology (5) | |
says co-author David Stuart, a structural biologist at the University of Oxford, UK, who is working with the World health organization
The first nanometer resolved image of individual tobacco mosaic virions shows the potential of low energy electron holography for imaging biomolecules at a single particle level--a milestone in structural biology and a potential new tool
Modern structural biology relies on techniques such as nuclear magnetic resonance (NMR), X-ray crystallography and cryo-electron microscopy to discover the tiny structural details of biomolecules.
single-particle imaging technique for structural biology. The researchers describe their work in a paper published this week on the cover of the journal Applied Physics Letters, from AIP Publishing."
"The virions are imaged with one nanometer resolution exhibiting details of the helical structure of the virus. Our technique would be the first non-destructive imaging tool for structural biology at the truly single molecule level."
The team employed cryo-electron microscopy, also known as CRYO EM, to observe the particles and origami frames. This work was headed by Huilin Li, Brookhaven Lab and Stony Brook University biologist,
CRYO EM preserves samples in their near-native states and provides close to nanometer resolution. We show that CRYO EM can be applied successfully to probe the 3d structure of DNA NANOPARTICLE clusters,
Wang said. The DOE Office of Science supported the study. DOE Office of Science supports the Brookhaven National Laboratory.
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