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The first nanometer resolved image of individual tobacco mosaic virions shows the potential of low energy electron holography for imaging biomolecules at a single particle level--a milestone in structural biology and a potential new tool
The work demonstrates the potential of low energy electron holography as a non-destructive, single-particle imaging technique for structural biology.
"We've shown that by means of low energy holography, it is possible to image individual tobacco mosaic virions deposited on ultraclean freestanding graphene,
Low energy electron holography is a technique of using an electron wave to form holograms. Similar to light optical holography
albeit with a much smaller wavelength, the complete information of the scattered wave, such as phase and amplitude, is recorded
"The low energy electron holography has two major advantages over conventional microscopy. First, the technique doesn't employ any lenses,
in low energy electron holography, the employed electron doses can be much higher--even after exposing fragile molecules like DNA or proteins to a electron dose more than five orders of magnitude higher
Sufficient electron dose in low energy electron holography makes imaging individual biomolecules at a nanometer resolution possible.
"Since low energy electron holography is a method very sensitive to mechanical disturbance, the current nanometer resolution could be improved to angstrom (one ten billionth of a meter)
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