Tropes

futurity_medicine 00551.txt

#Silly Putty component helps build carpet for stem cells The sponginess of the environment where human embryonic stem cells are growing affects the type of specialized cells they eventually become, a new study shows. The researchers coaxed human embryonic stem cells to turn into working spinal cord cells more efficiently by growing the cells on a soft, ultrafine carpet made of a key ingredient in Silly Putty. Their study is appears online in Nature Materials. This research is the first to directly link physical, as opposed to chemical, signals to human embryonic stem cell differentiation. Differentiation is the process of the source cells morphing into the body more than 200 cell types that become muscle bone, nerves, and organs, for example. Jianping Fu, assistant professor of mechanical engineering at University of Michigan, says the findings raise the possibility of a more efficient way to guide stem cells to differentiate and potentially provide therapies for diseases such as amyotrophic lateral sclerosis (Lou Gehrig disease), Huntington, or Alzheimer. In the specially engineered growth systemhe arpetsfu and his colleagues designedicroscopic posts of the Silly Putty component polydimethylsiloxane serve as the threads. By varying the post height, the researchers can adjust the stiffness of the surface they grow cells on. Shorter posts are more rigidike an industrial carpet. Taller ones are softerore plush. The team found that stem cells they grew on the tall softer micropost carpets turned into nerve cells much faster and more often than those they grew on the stiffer surfaces. After 23 days, the colonies of spinal cord cellsotor neurons that control how muscles movehat grew on the softer micropost carpets were four times more pure and 10 times larger than those growing on either traditional plates or rigid carpets. his is extremely exciting, Fu says. o realize promising clinical applications of human embryonic stem cells, we need a better culture system that can reliably produce more target cells that function well. Our approach is a big step in that direction by using synthetic microengineered surfaces to control mechanical environmental signals. Fu is collaborating with doctors at the University of Michigan Medical school. Eva Feldman, professor of neurology, studies amyotrophic lateral sclerosis, or ALS. It paralyzes patients as it kills motor neurons in the brain and spinal cord. Researchers like Feldman believe stem cell therapiesoth from embryonic and adult varietiesight help patients grow new nerve cells. She using Fu technique to try to make fresh neurons from patientsown cells. At this point, theye examining how and whether the process could work, and they hope to try it in humans in the future. rofessor Fu and colleagues have developed an innovative method of generating high-yield and high-purity motor neurons from stem cells, Feldman says. or ALS, discoveries like this provide tools for modeling disease in the laboratory and for developing cell-replacement therapies. The researchers verified that the new motor neurons they obtained on soft micropost carpets showed electrical behaviors comparable to those of neurons in the human body. They also identified a signaling pathway involved in regulating the mechanically sensitive behaviors. A signaling pathway is a route through which proteins ferry chemical messages from the cell borders to deep inside it. The pathway they zeroed in on, called Hippo/YAP, is involved also in controlling organ size and both causing and preventing tumor growth. Fu says his findings could also provide insights into how embryonic stem cells differentiate in the body. ur work suggests that physical signals in the cell environment are important in neural patterning, a process where nerve cells become specialized for their specific functions based on their physical location in the body, he says t

< Back - Next >