Synopsis: 4. biotech: Genus: Genetic vectors:


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and humans they mapped the DNA sequences on an evolutionary family tree for primates going back 60 million years estimating what genes could have looked like for extinct primate ancestors.


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We learned the genes in the body and now have a good understanding of how they cause disease

and gene silencing/overexpression to treat these. Stop being paranoid scientists are not elitists...We are little kids who still have the passion to ask why not.


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While thereã¢Â#Â#s little doubt that a personã¢Â#Â#s experiences and learned behaviors have a huge impact on their disposition it is also now widely accepted that genes


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When we translate material things from genes to jet planes into numbers we can analyze and manipulate them far more easily.


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Did you know the domesticated watermelon has about as many genes as you do? In 2012 genome researchers sequenced the DNA of an unborn human baby the western lowland gorilla fruits and grains and livestock.


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so that they have genes from a soil bacteria called Bacillus thuringiensis. The genes produce crystalline chemicals that kill insect larvae when they eat it.

A larva that chows down on a Bt-crystal-producing GM plant soon stops eating.


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The sequences of parent plants'genes represent some of the companies'most important intellectual property.


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To make Arctic apples biologists took genes from Granny smith and Golden delicious varieties modified them to suppress the enzyme that causes browning

Biologists also introduce genes to make plants pest-and herbicide-resistant; those traits dominate the more than 430 million acres of GMO crops that have already been planted globally.

Humans have been manipulating the genes of crops for millennia by selectively breeding plants with desirable traits.

They do this by either using bacteria to deliver the new genetic material or by shooting tiny DNA-coated metal pellets into plant cells with a gene gun.

or a toxin or if it is going to turn another gene off says Peggy G. Lemaux a plant biologist at the University of California Berkeley.

Genes that help wild plants survive might spread whereas those that say boost Vitamin a content might remain at low levels

Traditionally cheesemakers use rennet from the lining of cow stomachs to get their chymosin ut an estimated 80 to 90 percent of hard cheeses in the U s. are made with bacteria modified with the rennet-producing cow gene.

Today's most common GMO technology RECOMBINANT DNA inserts genes into a plant's cells via bacteria or specialized delivery tools but it involves some trial and error.

A new method called gene editing uses enzymes to snip out a specific bit of DNA to either delete it

Gene editing may also provide fodder for fresh controversy. Current GMO methods leave a trace behind

or example a bit of the DNA from bacterium used to insert new genes. The enzymes used in gene editing don't leave such a fingerprint so future genetically modified plants will be harder to detect with tests.

This article originally appeared in the July 2014 issue of Popular Science. Note (7/17/2014 6:


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mapping the birds gene sequences in order to determine the best approach for getting those good heat-resistant genes into American chickens without taking along all the genetic baggage as Schmidt calls it that s unnecessary to duplicate in the hybrid chickens.

Once Schmidt and his colleagues have deciphered and analyzed the genetic codes of these hardy African


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whose genes are similar to those found in closely related unmodified animals. So instead of giving pigs mouse genes scientists could make domestic pigs with genes normally found in wild pigs.

The end result would be engineered pigs that farmers could have made through generations of careful breeding geneticists argue.

I don't think those who don't wish to eat GM foods will find GM pigs-with-pig-genes any better than pigs with mouse genes.


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The filmmakers of Jupiter Ascending augmented human characters with animal genes to make them more physically imposing.

At other points it s a naturally occurring hiccup like the X-Gene that allowed superhumans to manipulate brain waves or magnetic fields.

Genes can mutate spontaneously or be manipulated in the lab to create new traits. Take for example the ability of most adult humans to process lactose in dairy products.


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and using genes from its host trees hich would explain why it mimics the nearest leaf


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and Blood Institute team specially engineered its pigs to have some human genes and to lack some pig genes.

The researchers also gave their baboons drugs to suppress their immune systems. Human patients take immunosuppressant drugs


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Second they inserted genes that code for the enzymes into the DNA of E coli (chosen


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if space affects which genes the staple expresses RIA Novosti reports. Updated February 3: Added comments from Bruce Bugbee c


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The technique called RNA interference or RNAI works by creating snippets of RNA that correspond to genes in the target species say corn rootworm.

When these chunks of genetic material enter the rootworm perhaps after being sprayed onto the crop the animal reacts to this RNA snippet as it would an invading virus. This prompts a response that attacks and silences the corresponding gene in the host's own DNA.

If this gene is necessary for the organism's survival the theory goes it dies.


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but it also helps reveal a few interesting secrets hiding within the pepper's genes. Because peppers are not so different from their cousins the potato

and we even know the gene that could turn it on and off Van Deynze tells Popular Science.

That gene is called capsaicin synthase. With the genome the team of researchers learned more about capsaicin synthase

The study suggests that the pungency from peppers was evolved through new genes by unequal duplication of existing genes.


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He found that there is little risk of genetically modified genes cross-pollinating with plants of other species

They likely arise from extensive herbicide use with Monsanto's Roundup Ready crops not directly from the Roundup Ready genes themselves.


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The same genes that make cows produce more milk also kill off cow embryos they found.

The European researchers found that up to a third of Nordic Red Cattle are missing one copy of each of the same four genes.

Like humans cows are supposed to have two copies of all of their genes one from their moms and one from their dads.

Having no copies of those four genes is deadly but at the same time having one copy of each makes a cow produce more milk than normal cows that have two copies.

Those missing genes are a gift with a dangerous catch. Of course before this study farmers didn't know how all of this worked.

thus passing on their shortened genes. That's why the genetic flaw is so common in Nordic cows.

whether the bulls they use in breeding are missing copies of these four genes Goutam Sahana a Danish geneticist who worked on the study said in a statement.

which may be missing those genes too. By preventing animals that are both missing genes from mating with each other a quantum jump in fertility could be achieved in Nordic Red breeds Sahana said.

What about cattle in the U s.?This study looked only at Nordic herds so it can't say


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Dzierson posited that males were haploid--possessing one set of chromosomes which was confirmed in the 1900s with the advent of the microscope.

The authors studied 14 natural sequence variants of the complementary sex determining switch (csd gene) for 76 genotypes of honey bees.

because they have one gene locus responsible for sex determination. Also Page and former graduate student Greg Hunt identified genetic markers--well-characterized regions of DNA--close to the complementary sex determining locus to allow gene mapping.

In addition Hunt and Page found that the honey bees'high recombination rate--the process by which genetic material is mixed physically during sexual reproduction--is the highest of any known animal studied

and Beye were also able to knock out an allele and show how one could get a male from a diploid genotype;

However the questions of which alleles were key how they worked together and in what combinations and why this system evolved were unanswered left

This compelled the current team of collaborators to step back to review what actually constitutes an allele.

There has to be some segment of that gene that is responsible in this allelic series where

if you have two different coding sequences in that part of the gene you end up producing a female said Page.

So we asked how different do two alleles have to be? Can you be off one

and look at these 18-20 alleles and find out what regions of these genes are responsible among these variants.

In this process we also had to determine if there are intermediate kinds of alleles and discover how they might have evolved said Page.

What the authors found was that at least five amino acid differences can control allelic differences to create femaleness through the complementary sex determiner (csd) gene--the control switch.

We discovered that different amounts of arginine serine and proline affect protein binding sites on the csd gene which in turn lead to different conformational states

which then lead to functional changes in the bees--the switch that determines the shift from female to not female said Page.


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Teasing out the hidden subtleties of a type of hybrid vigor involving just one gene has provided the scientists with means to tweak the length of time that bushy tomato varieties can produce flowers.

In his previous work CSHL Associate professor Zach Lippman and Israeli colleagues identified a rare example of hybrid vigor involving a genetic defect in the gene that makes florigen a hormone that controls the process of flowering

They found that bushy plants with a mutation in one of the two copies of the florigen gene producing half as much florigen as plants without the mutation do postpone the moment


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This reveals which genes--both parasite and host--are most active during infection. Most strikingly some South american and some atypical North american strains induced a type of immune reaction usually only seen during viral infection known as the type 1 interferon response.


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Some duplicated genes were lost over time but others took on new functions including contributions to the development of floral organs.

As another example of the value of the Amborella genome Joshua Der at Penn State noted We estimate that at least 14000 protein-coding genes existed in the last common ancestor of all flowering plants.

Many of these genes are unique to flowering plants and many are known to be important for producing the flower as well as other structures and other processes specific to flowering plants.

Jim Leebens-Mack from UGA noted that The Amborella genome sequence facilitated reconstruction of the ancestral gene order in the'core eudicots'a huge group that comprises about 75 percent of all angiosperms.

As an evolutionary outsider to this diverse group the Amborella genome allowed the researchers to estimate the linear order of genes in an ancestral eudicot genome

and function of protein-coding genes so the cessation of mobile DNA activity may have slowed the rate of evolution of both genome structure and gene function.


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In other words it was thought that female birds may have evolved similar patterning to males due to common genes but that female patterns would be lost subsequently as it is not beneficial.


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and analyse for the first time the sweet genes of beetroot. The results of the study that will be published today in Nature shed also light on how the genome has been shaped by artificial selection.

Information held in the genome sequence will be useful for further characterization of genes involved in sugar production and identification of targets for breeding efforts.

or quinoa as well as plants with an interesting biology for instance carnivorous plants or desert plants. 27421 protein-coding genes were discovered within the genome of the beet more than are encoded within the human genome.

Sugar beet has a lower number of genes encoding transcription factors than any flowering plant with already known genome adds Bernd Weisshaar a principle investigator from Bielefeld University who was involved in the study.

The researchers speculate that beets may harbor so far unknown genes involved in transcriptional control

and gene interaction networks may have evolved differently in sugar beet compared to other species. The researchers also studied disease resistance genes (the equivalent to the immune system in animals)

These genes turned out as particularly plastic with beet-specific gene family expansions and gene losses.

Additionally gene numbers varied between different sugar beet cultivars which contained up to 271 genes not shared with any of the other lines as Juliane Dohm

and Andr Minoche two scientists involved in the study commented. The researchers also performed an evolutionary analysis of each sugar beet gene in order to put them into context with already known genes of other plants.

This analysis allowed them to identify gene families that are expanded in sugar beet compared to other plants

but also families that are absent. Notably such gene families were associated most commonly with stress response

or with disease resistance added Toni Gabaldon group leader in the CRG Bioinformatics and Genomics programme and ICREA research professor.

and artificial selection gene regulation and gene-environment interaction as well as biotechnological approaches to customize the crop to different uses in the production of sugar


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and are able to retain expression of the inserted genes for at least 14 years a report in the Canadian Journal of Forest Research just announced.

A large-scale study of 402 trees from nine insertion events tracked the result of placing the cry3aa gene into hybrid poplar trees.

and reproduce in order to prevent any gene flow into wild tree populations researchers said. With this genetic modification the trees were able to produce an insecticidal protein that helped protect against insect attack.

Annual crops such as cotton and corn already are grown routinely as GMO products with insect resistance genes.

and why extended tests were necessary to demonstrate that the resistance genes would still be expressed more than a decade after planting.

Agencies are likely to require extensive studies of gene flow and their effects on forest ecosystems

Strauss said he advocates an approach of engineering sterility genes into the trees as a mechanism to control gene flow


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. In addition the mapping should help guide them to improvements in control methods and toward more usable genes and treatments.

which fall into an order to encode genes specific to an organism. Gabriel likened it to examining beads on a necklace:

and the color sequence determines each gene--in this case the DNA greening necklace held 1195201 beads or 1044 genes.


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Officially published Dec 1 in the journal G3 (Genes Genomics Genetics) the open-access research has been available online for several weeks and drawing global attention.

Chiu teamed with scientists at UC Davis Oregon State university the China National Gene Bank and the American Museum of Natural history as part of a $5. 8 million project on the biology and management of spotted wing drosophila funded by a U s. Department of agriculture Specialty Crops Research Initiative grant


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& Food Research and the University of Otago has identified the gene controlling bulb development the first step in discovering genetic markers that can be used as tools to screen conventional breeding programmes for new onion varieties with the right genetic


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Longer-term strategies focus on introducing genes to the Chinese oilseed rape plants to build disease resistance.


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#Genetic mutation increases risk of Parkinsons disease from pesticidesa team of researchers has brought new clarity to the picture of how gene-environmental interactions can kill nerve cells that make dopamine.

Research and professor of biology at the Massachusetts institute of technology (MIT) used skin cells from Parkinson's patients that had a mutation in the gene encoding a protein called alpha-synuclein.

The team plans to explore additional molecular mechanisms that demonstrate how genes and the environment interact to contribute to Parkinson's and other neurodegenerative diseases such as Alzheimer's and ALS.


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The analysis of the Rhizophagus irregularis genome has revealed that this asexual fungus doesn't shuffle its genes the way researchers expected.

Moreover rather than having lost much of its metabolic genes as observed in many mutualistic organisms it has expanded its range of cell-to-cell communication genes

and phosphorus-capturing genes. A team led by The french National Institute for Agricultural Research (INRA and including researchers from the Department of energy Joint Genome Institute (DOE JGI) reported the complete genome of R. irregularis (formerly Glomus intraradices) in a paper published online November 25 in the journal Proceedings of the National

Among the expanded portions of its genome R. irregularis had several genes for phosphorus metabolism

They also have an abundance of genes for communication between cells via signaling proteins including small secreted effectors highly expressed during symbiosis. Plant roots send out a plethora of chemical signals

and these genes probably help AMF interact with plants picking up the signals plants pump out.

Another surprise for the research team was in the genes that govern metabolism. Obligate parasites often have broken metabolism missing some genes in critical metabolic pathway

which make them dependent on their host Grigoriev said. We did not find such genes here.

R. irregularis has retained much of its metabolic machinery unlike many other obligate parasitic organisms. It leads a double-life extracting minerals from the soil

Though it has nearly 30000 protein-encoding genes R. irregularis has lost also hundreds of genes as a result of its close association with plants.

It has also cast off most of its genes for breaking down plant cell walls a critical ability for free-living fungi that feed off dead organic matter in soils.


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and exhibited extensive collinearity across the gene space species-specific genes involved in stress tolerance such as ion transport ATPASE activity transcript factor activity

When studying the adaptation mechanisms to against salt stress researchers found that several gene families likely to be involved in tolerance to salt stress contain significantly more gene copies in the P. euphratica lineage.

and found some genes involved in ion transport and homeostasis such as Nhad1 KUP3 and NCL were distinctly upregulated under salt stress.


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The results which also revealed potential treatment target genes are published in the November 2013 issue of The Journal of Cancer.

Further analysis also identified several key genes which could serve as markers to follow the progress of therapy.


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Previous studies had identified potential genes in L. johnsonii for producing EPS giving the researchers tools to probe how the bacteria synthesise these molecules.

Knocking out the whole cluster of EPS genes meant the bacteria produced no capsule. Further analysis of the genes by IFR Phd student Enes Dertli uncovered their potential roles in the capsule biosynthesis process

but more research is needed to fully understand the system and also how it is regulated. The structures of these two EPS molecules appear to be unique to this strain.


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We found a clear reduction of E coli strains possessing typical genes for extra-intestinal pathogenic E coli (Expec) says Bednorz.

because Expec typically harbor a lot of adhesion genes that promote colonization of the mucosa. Antimicrobials are thought to promote growth in industrially grown livestock


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Future research will focus on identifying the genes in the small intestine that are altered by the LPAS in order to find signaling pathways that may be targets for treatment.


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Researchers discovered that simple modifications to the drug furamidine have a major impact on its ability to affect specific human proteins involved in the on-off switches of certain genes.

The proteins--known as transcription factors--regulate the expression of genes in a highly coordinated and intricate manner making them attractive targets for therapeutic drugs


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When expressing the same phenotype various plant species apparently fall back on the same genes in their genotype.

The mutated gene carries the cryptic name GA20OX1. Maarten Koornneef and his colleagues from the Max Planck Institute for Plant Breeding Research in Cologne have examined now

whether Arabidopsis plants in the wild that grow to only half the height as other members of their same species also have mutated a GA20OX1 allele as the short rice

Using genetic crossbreeding experiments they have shown that this characteristic can be traced back to a change in the GA20OX1 gene in most of the plant specimens gathered.

This gene is especially interesting in Arabidopsis as mutations only cause the semi-dwarfism and have no further negative effects on the performance of the plants

The reason for this according to Koornneef is that Arabidopsis possesses other additional genes for gibberellin biosynthesis. These genes jump in

What changes cause Arabidopsis to switch the GA20OX1 gene off in the wild? Koornneef and his colleagues have identified six different genetic causes for the semi-dwarfism.

In addition the scientists have also found mutations that alter the reading frame of the GA20OX1 gene by removing part of the gene then lengthening the gene sequence through insertion of foreign DNA a so-called transposon.

The GA20OX1 gene is therefore one of these hot spots in the plants'genome becoming repeatedly mutated


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(or COS) and other antifungals and ARL1 gene inhibitor in inhibiting the growth of mold and yeast.

The chitosan or COS and a joint inhibition of some of its gene targets block the cell cycle


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and won't be around to pass on their genes the next summer.''Because small birds can't reproduce without surviving the winter they have evolved a complex set of behaviours that enables them to maximise their chance of both surviving predators


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#Gene responsible for hereditary cancer found to disrupt growth-regulating cellular pathwaywhitehead Institute scientists report that the gene mutated in the rare hereditary disorder known as Birt-Hogg-Dub cancer syndrome

In the case of Birt-Hogg-Dub syndrome the mutated gene prevents mtorc1 pathway activation early in the formation of tumors.

In the early 2000s scientists determined that mutations in the gene coding for FLCN caused the rare cancer Birt-Hogg-Dub syndrome

which means that a child inheriting one mutated copy of the FLCN gene will eventually develop the syndrome.

Usually diseases are described first then the responsible gene or genes are identified and then that gene's molecular function is figured out says Tsun.

And you need to know the gene's function before you can start working on drugs or therapy.

We've done that third step which is a very important discovery for these patients.

Story Source: The above story is provided based on materials by Whitehead Institute for Biomedical Research. The original article was written by Nicole Giese Rura.


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#Sex determiner gene of honey bee more complicated than thoughtbee colonies consist of a queen bee lots of female worker bees and some male drones.

The gene that determines the sex of the bees is much more complex than has been assumed up until now

In these fertilized eggs the condition of the complementary sex determiner (csd) gene is of crucial significance for the creation of female workers.

The queen bee who in the course of their mating flight mate with different drones multiple times passes on to fertilized eggs a random combinations of two csd copies so-called alleles.

If these alleles are different enough they develop into a female. If the csd gene in contrast is present in the fertilized eggs in two identical versions diploid drones develop.

These are eaten however by worker bees after they hatch. Up until now it was assumed that there were up to 20 csd alleles.

In the dataset which the research team under the direction of Hasselmann collected from all over the world

however 53 csd alleles found in localities in Kenya) and worldwide at least csd 87 alleles.

Using an evolutionary model the scientists extrapolated 116--145 csd alleles. New csd alleles were created in a relatively quick period for evolution: ca. every 400000 years.

A region inside the csd gene in particular represents a hot-spot with a high evolutionary rate that together with certain amino acid mutations decisively contributes to the formation of new csd alleles in the flanking regions.

The vitality of a bee population depends on amongst other things the genetic diversity of sex determining alleles.

These new findings are therefore very important for apiculture for minimizing the danger of inbreeding and thereby the production of diploid drones.


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With the aid of DNA markers we now know which gene is responsible for which molecular feature.


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#Surprises discovered in decoded kiwifruit genomea new study that decoded the DNA sequence of the kiwifruit has concluded that the fruit has many genetic similarities between its 39040 genes

when an extensive expansion of genes arose from an entire extra copy of the genome followed by extensive gene loss.

When genes are duplicated the extra genes can mutate to perform entirely new functions that were not previously present in the organism.

The duplication contributed to adding additional members of gene families that are involved in regulating important kiwifruit characteristics such as fruit vitamin C flavonoid

They uncovered about 8000 genes that were common among all five species. The comparison revealed important evolutionary relationships including the development genes related to fruit growth ripening nutrient metabolism and disease resistance.


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The basic idea of natural selection is to pass on your genes but Hamilton pointed out in an article that revolutionized the study of social evolution that you can pass on genes by helping your relatives as well as your offspring.

Hamilton predicted that organisms ought to evolve the ability to discriminate degrees of kinship more and more finely.

Genes that made them choosy would survive to future generations because the organisms would direct help to those individuals with whom they shared the most genes.

True say evolutionary biologists David Queller Phd the Spencer T. Olin Professor of Biology in Arts

But in others such as honeybee colonies the queen mates with multiple males and so there are 17 or 20 sets of paternal genes

Lifting the veil Hamilton predicted that kin recognition might reach all the way down to genes. He said it was possible that one gene might be able to detect other organisms carrying a copy of itself.

He was right. The red fire ant for example has a gene that worker offspring can recognize

and workers with this gene kill all queens that don't have it. Genes like this one are called greenbeards

because they confer an externally visible label that allows them to spot copies of themselves

much as if every man with a certain gene dyed his beard green. But what about the mother's genes and the father's genes in an embryo?

These long have been thought to lack self-identity and to be shrouded under their own veil of ignorance the assumption being that both sets of genes work for the common good.

We now know that some genes are marked as maternal or paternal through the addition of methyl groups while they are in the egg or sperm.

As a result of marking called imprinting the veil of ignorance is lifted. Considerable evidence suggests that imprinting leads to conflict between the matrigenes

and the patrigenes in the embryo Queller said. A mouse for example can mate with more than one male

It's in the interest of the father's genes to produce large offspring that compete for maternal resources at the expense of other pups.

And it's in the interest of the mother's genes that all of her offspring survive to adulthood

Not surprisingly many of the genes that are imprinted are involved somehow in growth and metabolism. The father's trying to crank up something that causes the offspring to get more


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