ScienceDaily_2013 07934.txt

#New possibilities for efficient biofuel productionlimited availability of fossil fuels stimulates the search for different energy resources. The use of biofuels is one of the alternatives. Sugars derived from the grain of agricultural crops can be used to produce biofuel but these crops occupy fertile soils needed for food and feed production. Fast growing plants such as poplar eucalyptus or various grass residues such as corn stover and sugarcane bagasse do not compete and can be a sustainable source for biofuel. An international collaboration of plant scientists from VIB and Ghent University (Belgium) the University of Dundee (UK) The James Hutton Institute (UK) and the University of Wisconsin (USA) identified a new gene in the biosynthetic pathway of lignin a major component of plant secondary cell walls that limits the conversion of biomass to energy. These findings published online in this week's issue of Science Express pave the way for new initiatives supporting a bio-based economy. This exciting fundamental discovery provides an alternative pathway for altering lignin in plants and has the potential to greatly increase the efficiency of energy crop conversion for biofuels said Sally M. Benson director of Stanford university's Global Climate and Energy Project. We have been pleased so to support this team of world leaders in lignin research and to see the highly successful outcome of these projects. Lignin as a barrierto understand how plant cells can deliver fuel or plastics a basic knowledge of a plant's cell wall is needed. A plant cell wall mainly consists of lignin and sugar molecules such as cellulose. Cellulose can be converted to glucose which can then be used in a classical fermentation process to produce alcohol similar to beer or wine making. Lignin is a kind of cement that embeds the sugar molecules and thereby gives firmness to plants. Thanks to lignin even very tall plants can maintain their upright stature. Unfortunately lignin severely reduces the accessibility of sugar molecules for biofuel production. The lignin cement has to be removed via an energy-consuming and environmentally unfriendly process. Plants with a lower amount of lignin or with lignin that is easier to break down can be a real benefit for biofuel and bioplastics production. The same holds true for the paper industry that uses the cellulose fibres to produce paper. A new enzymefor many years researchers have been studying the lignin biosynthetic pathway in plants. Increasing insight into this process can lead to new strategies to improve the accessibility of the cellulose molecules. Using the model plant Arabidopsis thaliana an international research collaboration between VIB and Ghent University (Belgium) the University of Dundee (UK) the James Hutton Institute (UK) and the University of Wisconsin (USA) has identified now a new enzyme in the lignin biosynthetic pathway. This enzyme caffeoyl shikimate esterase (CSE) fulfils a central role in lignin biosynthesis. Knocking-out the CSE gene resulted in 36%less lignin per gram of stem material. Additionally the remaining lignin had altered an structure. As a result the direct conversion of cellulose to glucose from un-pretreated plant biomass increased fourfold from 18%in the control plants to 78%in the cse mutant plants. These new insights published this week online in Science Express can now be used to screen natural populations of energy crops such as poplar eucalyptus switchgrass or other grass species for a nonfunctional CSE gene. Alternatively the expression of CSE can be engineered genetically in energy crops. A reduced amount of lignin or an adapted lignin structure can contribute to a more efficient conversion of biomass to energy. This research was financed co by the multidisciplinary research partnership'Biotechnology for a sustainable economy'of Ghent University the DOE Great lakes Bioenergy Research center and the'Global Climate and Energy Project'(GCEP. Based at Stanford university the Global Climate and Energy Project is a worldwide collaboration of premier research institutions and private industry that supports research on technologies that significantly reduce emissions of greenhouse gases while meeting the world's energy needs. Story Source: The above story is provided based on materials by VIB. Note: Materials may be edited for content and length. Journal Reference e


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